Simultaneous Determination of Contents of 6 Components in the Oil of Blumea balsamifera by GC / 中国药房

OBJECTIVE： To establish a method for simultaneous determination of contents of β-pinene， linalool， L-camphor， L-borneol， β-caryophyllene and xanthoxylin in the oil of Blumea balsamifera. METHODS： GC method was adopted. The determination was performed on RTX-1701 capillary column （programmed temperature）. The FID detector was controlled at 240 ℃. The inlet temperature was set at 240 ℃. The carrier gas was high-purity nitrogen 3 mL/min. The the sample size was 0.5 μL， and split ratio was 50 ∶ 1. RESULTS： The linear range of β-pinene， linalool， L-camphor， L-borneol， β-caryophyllene and xanthoxylin were 0.029 7-0.267 1 mg/mL （r＝0.999 9）， 0.024 3-0.218 9 mg/mL （r＝0.999 9）， 0.126 0-1.134 0 mg/mL （r＝0.999 9）， 0.217 2-1.954 8 mg/mL （r＝0.999 9）， 0.136 3-1.226 9 mg/mL （r＝0.999 9）， 0.044 5-0.400 3 mg/mL（r＝0.999 5）， respectively. The limits of quantitation were 0.028 5， 0.008 7， 0.018 6， 0.016 8， 0.014 5， 0.042 1 mg/mL； the limits of detection were 0.009 4， 0.002 9， 0.006 1， 0.005 5， 0.004 8， 0.013 9 mg/mL， respectively. RSDs of precision， stability， reproducibility and durability tests were all lower than 3％. The average recoveries were 98.13％-101.30％（RSD＝1.20％，n＝9），98.44％-101.81％（RSD＝1.28％，n＝9），98.26％-101.05％（RSD＝1.19％，n＝9），99.08％-101.58％（RSD＝0.89％，n＝9），98.66％-101.66％（RSD＝1.17％，n＝9），98.84％-103.60％（RSD＝0.96％，n＝9）， respectively. The contents of 6 components in the sample were 14.552-46.766， 16.951-22.096， 80.597-113.115， 205.224-242.537， 47.761-135.697， 26.493-45.771 mg/g， respectively. CONCLUSIONS： The established method is simple， accurate， precise and reproducible， which can be used for simultaneous determination of contents of 6 components in the oil of B. balsamifera. It can provide reference for comprehensive evaluation and extraction technology study of the oil of B. balsamifera.

Effect of high glucose on the expression of liver X receptors and ABCA1 in human THP-1 macrophages / 中华肾脏病杂志

Objective To investigate the effect of high glucose on the expression of liver X receptors (LXRs) and ATP-binding cassette transporter A1 (ABCA1) in human macrophages (THP-1 cell line). Methods THP-1 monocytes were differentiated into macrophages by induction of phorbol 12, 13-dibutyrate (PMA). Surface markers of macrophages were identified by CD68 immunohistochemistry. The macrophages were cultured with different concentration (5.6, 11.1, 22.2 and 33.3 mmol/L) of glucose and different time (0, 0.5, 2, 6, 12, 24, 48, 72 h). Real time PCR and Western blotting methods were used to examine the mRNA and protein expression of LXRs and ABCA1. Results As compared to 5.6 mmol/L glucose, macrophage LXRβ and ABCA1 were decreased significantly at both mRNA and protein levels in dose-and time-dependent manner (P<0.05). Conclusion Hyperglycemia may play a role in the pathogenesis of arteriosclerosis through the inhibition of LXRs and ABCA1 expression in diabetic patients.

Determination of the Dissolution of Qingyangshen Dispersible Tablets / 中国药房

OBJECTIVE:To establish a method for the determination of the dissolution of Qingyangshen dispersible tablets so as to provide method and parameters for quality control of this kind of tablets.METHODS:With water as dissolution medium in a rotating speed of 100 r?min-1,the dissolution of Qingyangshen dispersible tablets was determined by HPLC at a wavelength of 259 nm.RESULTS:Similar dissolution rates were obtained for 3 batches of Qingyangshen dispersible tablets,with accumulative dissolution rates of all samples at 45 minutes reached above 90%,and a good reproducibility was noted.CONCLUSION:The quality of Qingyangshen dispersible tablets is up to the standard stated in Chinese Pharmacopoeia(2005 Edition).

INVESTIGATION ON PRINCIPLE PARASITIC INFECTIONS IN THE SOUTHERN PART OF JIANGSU PROVINCE / 中国血吸虫病防治杂志

Objective To understand the present status of human parasitic infections and their characteristics in rural areas of the southern part of Jiangsu Province, to provide basis of making practical control measures.Methods Four thousand and eighty-two people were examined with stool tests and those people were distributed in 8 slected spots in the southern part of Jiangsu Province,according to the methods of national investigation scheme on human principal parasites. Results The avarage infection rate of parasites was 6.71%. The male and female infection rates of parasites were 4.77% and 8.42%,respectively. There were significant differences (P

Changes of Ascaris lumbricoides infection in rural residents of Jiangsu Province and its influencing factors / 中国血吸虫病防治杂志

Objective To investigate and analyze the changes of Ascaris lumbricoides infection in rural residents of Jiangsu Province during 13 years and its influencing factors in order to provide the basis of making practical policies for ascariasis control in future. Methods The data were analyzed about the rates of Ascaris infection and mass chemotherapy, per capita income, and the rates of the use of running water and hygienic toilets in the southern part, the middle part and the northern part of Jiangsu Province from 1990 to 2002, and the main influencing factors for Ascaris infection were explored. Results The prevalence rate of Ascaris infection decreased continuously during 13 years, from 39.51% to 2.14%, and the decrease rate was 94.58%. In 2002, the infection rate was the lowest, only 0.41%, decreased by 98.92% in the middle part of Jiangsu Province, and the infection rate was the highest, 5.09%, decreased by 89.97% in the northern part of Jiangsu Province. The accumulated rates of mass chemotherapy were 159.00% in middle part of Jiangsu Province,103.00% in the southern part of Jiangsu Province and 105.00% in the northern part of Jiangsu Prvince.The per capita income, the use of running water and hygienic latrines increased gradually from the northern part to the southern part and there were significant differences in the 3 survey regions. Conclusion The Ascaris infection rate is influenced by mass chemotherapy, economics and health conditions in rural residents, especially the mass chemotherapy.

Current status and analysis of intestinal nematode infection in Jiangsu Province / 中国血吸虫病防治杂志

Objective To understand and analyse the epidemic status of human intestinal nematode infection in Jiangsu Province, so as to provide basis for making control measures. Methods The typical thirty-nine spots in thirteen counties among the sampling spots of National Investigation on Distribution of Human Parasites in 1990 were selected and investigated according to the Methods of National Investigation Scheme on Human Principal Parasites in 2004. Results The total prevalence was 3.88%, which decreased by 46.92% compared with the investigation in 2002 (7.31%). The infection rates of hookworm, Ascaris and Trichuris were 1.14%, 1.06% and 1.73%, respectively, and the proportion of light infection was 91.75%. The rate of multiple infections was 9.40%. The proportion of hookworm infection was 78.06% in Southern Jiangsu and the proportion of Ascaris or Trichuris was 89.03% in Northern Jiangsu. The infection rate of Enterobius was 3.72% among children aged less than 12 years. Conclusions The prevalence of intestinal nematodes has decreased to lower level in Jiangsu Province. The difference of prevalence in different regions has a relationship with the disease control and social-economic, culture and health levels. The principal control work should be still put in the northern part of Jiangsu Province. The preschool children and the middle and old age peasants are high-risk population. The different control measures should be taken in accordance with the situation of different regions in the future.

Analysis of family clustering on principal human parasitic infections in Jiangsu Province / 中国血吸虫病防治杂志

Objective To understand the status and characteristics of principal human parasitic infections in rural areas of Jiangsu Province in order to provide basis for decision-making of practical control measures. Methods The survey data of principal human parasites in 58 sample sites of 19 counties (cities or areas) in Jiangsu Province were analysed on family clustering by G test, according to the methods of National Investigation Scheme on Principal Human Parasites. Results The infection of Ascaris lumbricoides, hookworm, Trichuris trichinra and Clonorchis sinensis in the province had obvious family clustering, while those in each county (city or district) appeared different states in some-points with the changing of parasitic infection rates and infection degrees. Conclusions The infection rates and infection degrees of principal human parasites decrease obviously in Jiangsu Province. The family clustering is disappearing with the dropping of them. Chemotherapy in family and health education should be strengthened to consolidate the control results in future.